Agarose gel comparing cloning results with the fermentas generuler 1 kb. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. The 1 kb dna ladder was not designed for precise quantification of dna mass, but can be. Lo department of microbiology and immunology, ubc ompa is. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. You really need to check the technical info that comes with the. Product information thermo scientific generuler 1 kb dna. The ladder is supplied with 6x dna loading dye, and it can be used in dna sizing and approximate quanitification applications.
The apparent molecular weight of each protein kda has been determined by. Migration patterns in different electrophoresis conditions using the prestained protein ladder v. This product is available in quickload purple format enjoy the convenience of a readytoload format. View more information about our dna stains technology. Electrophoresis of 100 to bp pcr products in agarose gel. In the pcr method, the dna fragments with length 102 bp lesser than the related. For descriptions of symbols on product labels or product documents, go to. The 1 kb dna ladder was not designed for precise quantification of dna mass, but can be used for approximating the mass of dna in comparably intense samples of similar size. Generuler 1 kb dna ladder, readytouse thermo fisher scientific. Investigating the effects of dmso on pcr fidelity using a. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restrict.
Thermo scientific generuler 100 bp dna ladder 100bp. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. Generuler 1kb dna ladder, range 250 to 10,000bpcan be labeled radioactively with t4 polynucleotide kinase seeprotocol. Hyperladder 1kb with 1% wv agarose gel, hyperladder 50bp with 1. Generating an epub file may take a long time, please be patient. Construction of a synthetic vector for preparation of a 100. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions. A molecularweight size marker, also referred to as a protein ladder, dna ladder, or rna. This marker is ideal for the size determination of digested dna.
A new genomic tool, ultrafrequently cleaving taqii. The dna includes fragments ranging from 25010,000 base pairs. Generuler 1 kb plus dna ladder thermo fisher scientific. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. These ladders are also available in a readytouse format with tritrack loading buffer premixed with the dna solution.
Construction of pbad24ompa for modulating ompa expression in. Construction of pbad24ompa for modulating ompa expression in escherichia coli to assess role in conjugation bernard c. Generuler 1 kb dna ladder thermo fisher scientific. Thermo scientific generuler 1 kb dna ladder 1kb dna ladder. Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis. If samples need to be diluted, use te or other buffer of minimal ionic strength. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb.
Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the product specification sheet, certificate of analysis, data card or product manual. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. In a 1 kb ladder, the bands are 1kb apart in size, though ones at the lower end may be closer in size than 1kb. The dna ladder consists of proprietary plasmids which. They cover a broad range of dna lengths and can be used to assess the dna amount per band. Biochain provides dna molecular weight markers for the determination of the dna fragment, such as pcr amplicon, restriction enzyme digested fragment, precisely on agarose gel. The 1 kb and 3 kb bands contain more dna to provide internal orientation.
The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. This marker can be used with either agarose or polyacrylamide gels. A novel combined method for costbenefit production of dna ladders. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Identification of the preparationsubstance and of the company preparation name. The ladder consists of bands that are generated from pcr and restriction enzyme. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel. For longer periods store at 20generuler 1 kb dna ladder, c. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and.
Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. When stored under the recommended conditions, the productis stable for 2 years. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. Ready to load ladders contain highly purified pcr products combined with plasmid digests, they are supplied in 1x. Available on gsava contract for federal government customers. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp.
Introduction ii protein molecular weight and reference standards 1. For gels with other lane widths, the components of the mixture should be scaled either up or down. Dna ladder 1 l 6x tritrack dna loading dye 1 l deionized water 4 l 6 l step 1. Thermo scientific generuler 1 kb dna ladder, readytouse. Readytouse 1 kb dna ladder and readytouse 100 bp dna.
Pdf straightforward procedure for laboratory production of dna. Specifications and individual lot data from the tests that are performed for. Thermo scientific generuler 1kb dna ladder, readytouse. Hyperladder 1kb is a popular, readytouse, molecular weight marker. Lo department of microbiology and immunology, ubc ompa is an abundant outer membrane protein in escherichia coli that has been shown to play an important role in bacterial conjugation by maintaining membrane stability. Quality control tests are performed on each new lot of neb product to meet the specifications designated for it. How can i prepare working solution with 1 kb dna ladder. Construction of a synthetic vector for preparation of a. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield. Generuler 1 kb, 100 bp, 50 bp, mix and express dna ladders.
Dna ladder 1 l 6x dna loading dye 1 l deionized water 4 l 6 l step 1. A molecularweight size marker in the form of a 1kb dna ladder in the rightmost lane, used in gel electrophoresis. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. These universal ladders are for dna sizing and approximate quantification. Pdf dna ladder is commonly used to determine the size of dna fragments by. The bands of the ladder each contain from 1 to 12 repeats of. Gel conditions are 1% agarose, 3 voltcm, and ethidium bromide stain. The ladder is supplied with 6x dna loading dye, and it. The ladder is a mixture of chromatographypurified individual dna fragments. Thermo scientific generuler 1 kb dna ladder is designed for. Description the dna ladders are ladders with defined sizes. The ppsu plasmids for generating dna molecular weight markers.
Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. You really need to check the technical info that comes with the ladder for the details on each product, as some have different product names which mean the same thing. Genomics and metagenomics are currently leading research areas, with dna sequences accumulating at an exponential rate. This ladder has 12 evenly spaced bands ranging from 1kb. Thermo fisher scientific freezer 2016 university of guelph. Feb 15, 2012 in a 1 kb ladder, the bands are 1kb apart in size, though ones at the lower end may be closer in size than 1kb. The 100 bp and 1kb dna ladders are used frequently in molecular biology as. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. Biochain provides dna molecular weight markers for the determination of the dna fragment, such as pcr amplicon, restriction enzyme digested.
How can i prepare working solution with 1 kb dna ladder supplied by promega. The readyto use dna ladders are supplied in a readytoload format at an. Shop online for a wide selection of thermo scientific generuler 1 kb plus dna ladder perform sizing and approximate quantification of a wide range of. The extra bands at 1 kb are present due to start activity and may be reduced with shorter incubation time. Generuler 100 bp dna ladder can be labeled radioactively with t4 polynucleotide kinase. The ladder consists of fragments ranging in size from 250 to 10,000 base pairs. Generuler 1 kb dna ladder, readytouse 250 5 x 50 g for 500 applications, 0. In addition to these 12 bands, the ladder contains vector dna fragments that. Both 100 bp and 1 kb ladders can either be purchased from commercial sources or generated in the laboratory. If the ionic strength of the sample is too low, blurring of the bands can occur. This 1kb dna ladder is a convenient marker for determining the size of doublestranded dna from 250 to 10,000 base pairs.
Effect of dmso in the pcr on the pattern of restriction fragments generated by digesting the pcr product of lacz with various restriction enzymes. Description the ladder is a mixture of chromatographypurified individual dna fragments. The product is supplied in a solution of 10 mm trishcl, ph 8. The 1 kb dna ladder is a readytoload molecular weight marker suitable for dna fragment size determination on gel electrophoresis. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna.
The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. Thermo scientific generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb. Load on the gel for gels with other lane widths, the components of the mixture should be scaled either up or down. Migration patterns of pageruler prestained protein ladder in different electrophoresis conditions. Supplementary data should be considered for more accurate adjustment. Invitrogen 1kb dna extension ladder 100ug nucleic acid. Invitrogen 1 kb plus dna ladder nondistribution product as customer accommodation. Dna ladder 100 bp 1kb marker for agarose gel electrophoresis. Please have a look into the related application note for more information about ladder migration in different gel concentrations. Load the same volumes of the dna sample and the dna ladder.